Removal of Cytotoxicity From A Mangosteen Polar Antioxidant Extract By Gel Filtration Chromatography

Fitri Rahmayanti, Miranti Miranti, Hilwan Yuda Teruna, Titania Tjandrawati Nugroho

Abstract


Mangosteen (Garcinia mangostana) fruit pericarp contain antioxidant compounds.  A concentrated extract of Garcinia mangostana fruit pericarp in 50% ethanol and 0.05M NaOAc pH 5,5 buffer, had an in vitro antioxidant activity (IC50) of 43.7 µg/ml, when measured by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method.  However, this concentrated extract was toxic when applied to keratinocyte cells, with IC50 of 5.3 µg/ml.  To eliminate the toxic compounds from the antioxidant activity, the extract was fractionated by the gel filtration column chromatography method, using Sephadex LH-20.  The column was eluted with methanol, and each  25ml of the eluted filtrate collected. The fractions were then concentrated by vacuum rotary evaporation and dried to constant weight at 50oC. Three fractions gave significantly higher in vitro DPPH antioxidant activity than before fractionation.  Fraction 2, 3 and 7 had IC50 of 13 µg/ml, 19 µg/ml and 11 µg/ml respectively.  Cytotoxicity testing using the Brine Shrimp Lethality Test showed that fraction 3 and 7 had significantly reduced cytotoxicity compared to the cytotoxicity of the crude extracts before gel filtration.


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